The invention discloses an enteral nutritional food as well as a preparation method and application thereof. The enteral nutrition food comprises 10 to 40 parts of isolated albumin powder, 5 to 18 parts of freeze-dried fruit powder, 5 to 20 parts of enzymolysis oat flour, 5 to 15 parts of MCT powder, 5 to 18 parts of whole milk powder, 10 to 30 parts of maltodextrin, 5 to 18 parts of microwave puffed grains, 3 to 10 parts of coconut powder, 2 to 8 parts of Ceylon instant black tea powder, 3 to 15 parts of solid malt extract, 2 to 5 parts of low-methoxy pectin, 1 to 12 parts of fructo-oligosaccharide, 1 to 5 parts of L-glutamine, 0.6 to 4 parts of arginine, 3 to 20 parts of soybean oligopeptide, 0.71091 to 2.41419 parts of compound vitamin and 0.29103 to 0.52907 parts of compound minerals.After enrichment of the varieties of enteral nutritional foods, such as microwave puffed grains, enzymolysis oat flour, freeze-dried fruit powder, sugar-free coconut powder and inulin, on the one hand, the stomach discomfort of a patient caused by food source change in a short time is relieved, the satiety is enhanced, and the stomach function recovery is promoted; and on the other hand, the gastrointestinal compliance of the patient is improved, the digestive absorption function is enhanced, the nutritional status of the patient is improved, and the hospitalization time is shortened.