13 results about "Peptide analysis" patented technology

The present disclosure relates to methods for high-throughput analysis of proteins and peptides employing a set of engineered binders that recognize terminal amino acid residues of peptide analytes. In particular, disclosed herein is the analysis of occupancy of N-linked glycosylation sites in proteins and peptides. The disclosure finds utility at large-scale profiling of N-linked glycosylation sites, as well as monitoring changes in glycosylation patterns associated with numerous disease conditions.

In the analysis method according to the present invention, a predetermined peptide is separated by immunoprecipitation using an antibody which specifically binds to either an N-terminus or a C-terminus of the predetermined peptide. The separated predetermined peptide is digested with a protease to prepare peptide fragments, and among the peptide fragments, a peptide fragment at a terminus opposite to a terminus binding to the antibody is mass-spectrometrically detected.

The present disclosure relates to methods for high-throughput analysis of proteins and peptides employing a set of engineered binders that recognize terminal amino acid residues of peptide analytes. In particular, disclosed herein is the analysis of occupancy of N-linked glycosylation sites in proteins and peptides. The disclosure finds utility at large-scale profiling of N-linked glycosylation sites, as well as monitoring changes in glycosylation patterns associated with numerous disease conditions.

The invention relates to the technical field of processing and application of bioactive peptides, and particularly discloses a preparation method of suaeda salsa oligopeptide and a determination method of sequence composition of the suaeda salsa oligopeptide, the molecular weight of the suaeda salsa oligopeptide is less than 2KDa, and the suaeda salsa oligopeptide contains 48 peptide fragment sequences and has antioxidant activity and in-vitro lipid lowering effect; crude protein is extracted from suaeda salsa and separated to obtain suaeda salsa oligopeptide, the sequence composition of the suaeda salsa oligopeptide is analyzed and determined, and the antioxidant activity and the in-vitro lipid-lowering effect of the suaeda salsa oligopeptide are detected. Compared with the suaeda salsa crude protein, the oligopeptide has good biological activity, is easier to absorb and utilize, and can be widely applied to the industries of medicines, special medical foods, health care products and the like.

This application discloses a method for determining the standard value of neuron-specific enolase (NSE) solution, belonging to the field of biotechnology. The determination method provided in this application includes characterizing the physicochemical properties of the NSE standard candidate solution, such as purity, molecular weight, secondary structure, and bioactivity; diluting and dispensing the NSE standard candidate solution; accurately determining the content of the NSE standard candidate solution and verifying its homogeneity and stability using isotope dilution mass spectrometry based on amino acid analysis and characteristic peptide analysis; and evaluating the uncertainty of the NSE solution standard value determination results. The determination method provided in this application has good reliability and accuracy, and the determination results are traceable to SI units (mol).

The application discloses a short peptide extracted from Epimedium and application of the short peptide in promoting bone growth. The short peptide is extracted from traditional Chinese medicinal material Epimedium, and a mechanism of the short peptide for improving bone-related diseases is analyzed. It is found that a short peptide with a sequence of VPIVMHDYLTGGFTANTSL can promote proliferation of osteoblasts by activating a phosphorylation pathway of MAPK, thereby laying a certain foundation for research on a new drug for improving bone-related diseases.

Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.

In some embodiments, methods for increasing polypeptide sequence coverage include removing inter-chain disulfide bonds from a first sample and retaining substantially all intra-chain disulfide bonds, and performing a first mass spectrometry (MS) analysis on the first sample to determine a first amino acid sequence, the interchain and intrachain disulfide bonds of the second sample are removed and a second MS analysis is performed to determine a second amino acid sequence, and the first and second sequences are combined to determine a combined amino acid sequence of the polypeptide.

The application discloses a preparation method and application of a novel composite material of magnetic core-porous hydrophilic MOFs for serum glycopeptide analysis. First, based on the in-situ MOF growth strategy, the composite material with a magnetic inner core and a multistage-pore MOFs shell is synthesized. Then, the composite material is used for glycopeptide separation and enrichment of a target sample, and then MALDI-TOF MS analysis and LC-MS / MS analysis are carried out. The combination of the composite material and the robust enrichment process can realize ultra-low detection limit (1 amol / μL) and ultra-high repeatability (CV<15%) of glycopeptides, and the finally detected serum glycopeptides can be further used for screening of disease-related differential expression markers.

Devices are provided for multiplex immunopeptide analysis from a small number of cells, scarce human tissue samples and biopsy, the devices comprising a microfluidic chip having at least two mirrored microfluidic channels connected in series for simultaneous enrichment of tumor-specific immunopeptides by a single sample injection. The invention also provides a method for preparing the device and a method for carrying out multiple immune peptide analysis by using the device.

The invention provides a polypeptide composition with an effect of reducing blood pressure as well as a preparation method and application of the polypeptide composition, the polypeptide composition comprises a polypeptide LVV-Hemorphin-6 and a polypeptide VV-Hemorphin-7, and the polypeptide composition is obtained by separating intracellular metabolites of a Brevibacterium sp. MTL5-77 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 18765. The preparation method comprises the following steps: firstly, extracting active ingredients of brevibacterium MTL5-77 subjected to fermentation culture, then carrying out primary purification by virtue of an anion exchange chromatography technology so as to remove impurities, then carrying out purification twice by virtue of a reversed-phase high-performance liquid chromatography, and finally, carrying out fine purification by virtue of a Posisil ODS-P polypeptide analysis column, so as to obtain the brevibacterium MTL5-77. Therefore, the polypeptide composition is successfully separated and obtained. The polypeptide composition is derived from marine strains, and a new strain resource is provided for research and development of polypeptide drugs for treating hypertension.

The present disclosure relates to a metalloprotein binder that specifically binds to a N-terminally modified peptide. Also provided herein is methods and related kits for treating or analyzing a peptide using the metalloprotein binder. The methods and compositions provided herein are useful for high-throughput peptide analysis and / or sequencing.

To provide sensitive and selective assay methods for differentiating full-length HMWK and cleaved HMWK.SOLUTION: A method for detecting cleaved high molecular weight kininogen (HMWK) in a sample comprises: (i) preparing a sample suspected of containing HMWK; (ii) contacting the sample with a protease to generate a plurality of digested peptides; and (iii) measuring the level of a signature peptide in the plurality of digested peptides, where the signature peptide is indicative of cleaved HMWK.SELECTED DRAWING: Figure 7