38 results about "Sinus Node Diseases" patented technology

Cell transplantation for constructing biological heart pacemaker is used embryo source dry / ancestral cell and adult mesogalia dry cell as cell sources. The process is carried out by oriented differentiation inducing for heart pacemaker cell by embryo source multifunctional dry cell, bone marrow adhering to obtain primary culture cell, clone culturing to obtain purifying system by diluting, applying paracrine factor endothelin-1 or nervous adjusting protein-1, extracellular matrix laminated adherent protein and fiber connecting protein etc. substrate glue, trans-dyeing pacemaker gene HCN4, and in-vitro inducing embryo source multifunctional dry cell. It is various and non-immunogenicity. It can be used to treat sick sinus syndrome.

The present invention relates to novel compositions and methods to induce, and / or modulate bio-electrical rhythms (e.g. in cardiac, neuronal and pancreatic cells) by fine-tuning the activity of HCN-encoded pacemaker channels via a novel protein- and genetic-engineering approach to augment or attenuate the associated physiological responses (e.g. heart beat, neuronal firing, insulin secretion, etc) for achieving various therapeutic purposes (e.g. sick sinus syndrome, epilepsy, neuropathic pain, diabetes, etc).

The invention discloses a traditional Chinese medicine composition for treating sick sinus syndrome. The traditional Chinese medicine composition is prepared from radix astragali, honey-fried herba ephedrae, radix codonopsis, cornus officinalis, deerhorn glue, psoralea corylifolia, epimedium, tuber of dwarf lilyturf, radix scrophulariae, ligustrum lucidum, wolfberry, ligusticum wallichii, bitter orange, turmeric root tuber, lumbricus, licorice and monkshood. The traditional Chinese medicine composition can be used in clinic treatment of sick sinus syndrome.

The invention relates to a medicament for treating sick sinus syndrome, in particular to a traditional Chinese medicine decoction for treating sick sinus syndrome. The traditional Chinese medicine decoction is prepared from the following raw materials in parts by weight: 9-30 parts of astragalus root, 6-12 parts of rhizoma anemarrhenae, 3-10 parts of bupleurum, 3-10 parts of balloonflower, 3-10 parts of cimicifuga foetida, 9-30 parts of dangshen, 5-20 parts of fructus corni, 3-10 parts of cassia twig and 1-10 parts of honey-fried licorice root. The traditional Chinese medicine decoction is prepared by the steps of: putting the raw materials into a clean container, adding cold water and soaking for half an hour, boiling with strong fire, then keeping a slight boiling state with soft fire and decocting for half an hour, then pouring out the decoction and filtering; adding cold water into the container again to submerge the medicinal materials for 3-5 centimeters, boiling with strong fire, then keeping the slight boiling state with soft fire and decocting for half an hour, then pouring out the decoction and filtering, and mixing with the previously poured out decoction. Compared with the prior art, the traditional Chinese medicine decoction provided by the invention has the advantages of reliable curative effect, no toxic side effects and simple preparation process.

The invention discloses a traditional Chinese medicine composition for treating cold blood stasis type sick sinus syndrome. The traditional Chinese medicine composition is prepared from codonopsis pilosula, rhizoma atractylodis macrocephalae, poria cocos, Chinese yam, astragalus membranaceus, ginseng, trogopterus dung, morinda officinalis, deer-horn glue, pollen typhae, flos carthami, polygonum cuspidatum, the root of straight ladybell, radix ophiopogonis, schisandra chinensis, angelica sinensis, radix polygonati officinalis, polygonatum kingianum, rhizoma cimicifugae, rhizoma chuanxiong, the root of red-rooted salvia, the root of common peony, peach kernel, radix curcumae, prepared aconite root, fructus psoraleae, pimpinella thellungiana, herba epimedii, rhizoma cyperi, fructus aurantii, fourstamen stephania root, fructus trichosanthis, pinellia ternate, arisaema consanguineum schott, bamboo juice, orange peel, ephedra, cassia twigs, notopterygium root, olibanum, myrrh, asarum and radix glycyrrhizae. By means of the traditional Chinese medicine composition, cold blood stasis type sick sinus syndrome can be safely and effectively treated.

The invention discloses a medicine for treating slow arrhythmia sick sinus syndromes and a preparation method thereof. The active components of the medicine for treating slow arrhythmia sick sinus syndrome are prepared from the following raw materials in weight proportion: 1 to 90g of red ginseng, 1 to 90g of processed Radix Aconiti Lateralis, 1 to 90g of Radix Glycyrrhizae Preparata, 1 to 90g of cassia twig, 1 to 90g of Panax pseudo-ginseng and 1 to 90g of Poria cocos. The medicine is obviously superior to atropine in the aspect of improving clinical symptoms, can improve the heart functions of patients and improve the quality of life of the patients; the medicine is superior to the atropine in the aspects of increasing the heart rate and therapeutic effect indexes of Holter, electrocardiogram and the like, improving EF and CO values, an electrocardiogram ST segment and the like in the ultrasonic heart function indexes of hearts; obvious adverse effects are not generated in treatment groups in the research process, and the medicine is clinically safe and valid. Therefore, the medicine has huge popularization and application values in the field of treating the slow arrhythmia sick sinus syndromes.

The invention relates to a meglumine adenosine cyclophosphate powder injection medicine composition for injection and a preparation method thereof. Specifically, the invention relates to a medicine composition on one hand and the medicine composition comprises adenosine cyclophosphate, meglumine, mannitol and an optional acid-alkali regulator, wherein based on every 10 parts of adenosine cyclophosphate by weight, the medicine composition comprises the following materials in parts by weight: 5.5-6.5 parts of meglumine, 9-15 parts of mannitol and a proper use amount of the acid-alkali regulator; and the use amount of the acid-alkali regulator ensures that when the medicine composition is dissolved and diluted in water to prepare a solution every 1ml of which contains 10mg of adenosine cyclophosphate, the pH value of the solution is 5.5-7.0. The powder injection composition disclosed by the invention can be used for treating cardiac failure, myocarditis, sick sinus syndromes, coronary heart diseases and cardiomyopathy and can also be applied to adjuvant therapy of cardiac arrhythmia; and the meglumine adenosine cyclophosphate powder injection medicine composition for injection disclosed by the invention has good pharmaceutical properties.

A cardiac monitoring device for determining the occurrence of a sick sinus syndrome condition of a patient that includes electrodes to sense a cardiac signal, a sensing module electrically coupled to the electrodes having circuitry positioned therein to receive the sensed cardiac signal, and a processor coupled to the sensing module and configured to determine an RR interval variability during an RR interval variability session in response to the sensed cardiac signal, determine whether a P-wave occurs during the RR interval variability session, determine whether a sick sinus indicator is satisfied in response to a P-wave occurring, increment a sick sinus count in response to the sick sinus indicator being satisfied, determine whether a sick sinus burden is satisfied in response to the sick sinus count being incremented, and determine the occurrence of sick sinus syndrome in response to the sick sinus burden being satisfied.

The invention discloses a traditional Chinese medicine composition for treating sick sinus syndrome. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-5 parts of roasted ephedra, 2-5 parts of asarum, 10-20 parts of pinellia ternate, 10-20 parts of radix liquiritiae, 8-15 parts of processed radix aconiti lateralis, 8-15 parts of rhizoma curculiginis, 8-15 parts of herba epimedii, 8-15 parts of fructus schizandrae, 10-20 parts of radix codonopsis, 10-20 parts of astragalus, 20-40 parts of crude dragon bones, 20-40 parts of raw oyster shells, 8-15 parts of polygala tenuifolia, 20-40 parts of salviae miltiorrhizae and 20-40 parts of semen platycladi. The traditional Chinese medicine composition has the effects of tranquilizing mind by nourishing the heart, tonifying kidneys to assist heart-yang and resisting arrhythmia, can eliminate atrionector impulse disorder and impulse conduction disorder from atrionector to atrium cordis, realizes the purpose of treating sick sinus syndrome, and has the advantages of being remarkable in effect, stable in curative effect and free of toxic and side effect.

The invention discloses a traditional Chinese medicine composition for treating bundle branch block and related heart diseases. The traditional Chinese medicine is made by combining Chinese herbal formulas of monkshood, dried radix rehmanniae, polygonum multiflorum, scrophulariae, salviae miltiorrhizae, red peony roots, raw hawthorns, rhizoma chuanxiong, cassia twig, raw fossil fragments, raw oyster shells, earthworms, amomum, rhizoma alismatis, astragalus, ephedra, radix glycyrrhizae, pulp of cornus and the like. The traditional Chinese medicine composition integrates tonifying and dredging and has the effects on promoting blood circulation and removing blood stasis, promoting circulation of qi and blood, enhancing metabolism and repairing important viscera trauma, and promoting metabolism and regeneration of brain cells, liver cells and myocardial cells. Heart system diseases comprise tachycardia, bradycardia, premature beat, atrial fibrillation and flutter, atrioventricular block, branch conduction block, sick sinus syndrome, pre-excitation syndrome, heart failure, carditis, pericarditis, part of neurosis and the like. The tradition Chinese medicine composition has the miracle effect on treating stagnation of the heart-blood, chest obstructions and chest pains and has the good curative effects on repairing rheumatic heart disease cardiac muscle valves and treating congenital heart disease valve defects and cardiac muscle inferior myocardial infarction.

The invention discloses a traditional Chinese medicine composition for treating Qi deficiency and blood stasis type sick sinus syndrome. The traditional Chinese medicine composition is prepared from astragalus membranaceus, radix pseudostellariae, poria cocos, codonopsis pilosula, morinda officinalis, cornu cervi degelatinatum, herba epimedii, radix polygonati officinalis, rhizoma acori graminei, the root of common peony, donkey-hide gelatin (molten by heat and taken after being mixed with water), ligusticum wallichii, angelica sinensis, the root of red-rooted salvia, trichosanthes kirilowii maxim, fructus aurantii, red ginseng, arisaema cum bile, mukdenia rossii, vaccinium bracteatum thunb, flos carthami, pseudo-ginseng, silene conoidea, trogopterus dung, the root of kudzu vine, schisandra chinensis, radix ophiopogonis, gastrodia elata, rhodobryum roseum, lumbricus, ephedra, cassia twigs, radix aconiti lateralis preparata, asarum, polygala tenuifolia, dens draconis, nacre mother of pearl, dried radix rehmanniae, raw dragon bone, tuber fleeceflower stem, raw oyster shell, spina date seed, tuber fleeceflower stem, honeysuckle, dandelion, jasmine, fructus psoraleae, rhizoma zingiberis and honey-fried licorice root. By means of the traditional Chinese medicine composition, Qi deficiency and blood stasis type sick sinus syndrome can be safely and effectively treated.

The invention relates to a traditional Chinese medicine preparation for treating a sick sinus syndrome. The traditional Chinese medicine preparation comprises agilawood, herba epimedii, radix aconite lateralis preparata, red ginseng, liquorice, schisandra chinensis, antlet glue, ginseng, asarum, fructus aurantii immaturus, light wheat, nacre, platycladi seed, polygonatum kingianum, motherwort, herba Inulae, tatarian aster root and angelica sinensis, has the efficacies of warming heart-yang, benefiting heart qi, warming and smoothening blood vessels, invigorating the circulation of blood and strengthening the heart, is capable of stimulating sinoatrial node and atrioventricular node of the heart, increasing the heart rate, expanding coronary arteries, improving blood supply of sinoatrial node, exciting sympathetic nerves, increasing the cardiac output, and enhancing the myocardial contractility, so that the blood circulation is improved; the blood system restores the self-adjusting function; through comprehensive treatment, the symptoms such as faint, palpitation, shortness of breath, dim complexion, dizziness, tinnitus, aching lumbus, limp knees, dysphoria, hypomnesis, insomnia, dizziness, palpitation, congestive heart-failure and angor pectoris can be thoroughly relieved; finally the yin-yang balance is reached; and the effective healing target of the sick sinus syndrome is reached.

The invention discloses a traditional Chinese medicine preparation for treating a sick sinus syndrome, which comprises the following active ingredients by weight: 20-30g ramulus cinnamomi, 10-20g agilawood, 10-20g herba epimedii, 10-20g red ginseng, 10-20g herba ephedra, 10-20g fructus aurantii immaturus, 10-20g semen platycladi, 10-20g pinellia ternata, 8-12g herba inulae, 8-15g aster, 8-12g calamus, 8-12g roasted rhizoma atractylodis macrocephalae, 8-12g roasted polygala tenuifolia willd, 8-12g baked ginger, 8-12g schisandra chinensis, 8-12g rohdea japonica var. variegata, and 8-12g carthamus tinctorius. The active ingredients are excellent in compatibility, and exert effects of supporting body resistance, tonifying qi, warming yang, promoting qi and blood, promoting blood circulation, strengthening a heart, nourishing the heart and restoring a pulse. The traditional Chinese medicine preparation for treating the sick sinus syndrome has the advantages of definite curative effect, high cure rate, no toxic or side effect, low price and the like, and is worthy of clinical popularization and application.

The invention relates to a sick sinus syndrome and a drug for treating the same. According to the viewpoint of traditional Chinese medicines, the sick sinus syndrome is analyzed to belong to heart-kidney yang deficiency, qi and blood deficiency as well as wind-phlegm blocking collaterals and is treated by using a traditional Chinese medicine composition with the effects of nourishing and warming kidney-yang, tonifying qi and lifting yang, cooling blood and clearing heart, dispelling wind and reducing phlegm as well as stimulating the circulation of the blood and causing the muscles and joints to relax. The drug is prepared from 15 parts of radix codonopsis, 15 parts of salvia miltiorrhiza, 15 parts of astragalus, 7 parts of acorus calamus, 20 parts of radix rehmanniae, 15 parts of radix ophiopogonis, 15 parts of dragon teeth, 20 parts of alocasia, 15 parts of honeysuckles, 15 parts of safflowers, 20 parts of Chinese wax gourd peel, 10 parts of lucid ganoderma, 8 parts of wolfberry and 7 parts of liquorice. The drug is remarkable in taking effect, capable of treating both symptoms and root causes and stable in curative effect.

Levosimendan, or (−)-[[4-(1,4,5,6-tetrahydro-4-methyl-6-oxo-3-pyridazinyl)phenyl]hydrazono]propanedinitrile, which has been previously suggested for the treatment of congestive heart failure is useful in the treatment of sick sinus syndrome and / or sinoatrial conduction disorders.

Cell transplantation for constructing biological heart pacemaker is used embryo source dry / ancestral cell and adult mesogalia dry cell as cell sources. The process is carried out by oriented differentiation inducing for heart pacemaker cell by embryo source multifunctional dry cell, bone marrow adhering to obtain primary culture cell, clone culturing to obtain purifying system by diluting, applying paracrine factor endothelin-1 or nervous adjusting protein-1, extracellular matrix laminated adherent protein and fiber connecting protein etc. substrate glue, trans-dyeing pacemaker gene HCN4, andin-vitro inducing embryo source multifunctional dry cell. It is various and non-immunogenicity. It can be used to treat sick sinus syndrome.

The invention discloses a traditional Chinese medicine composition for treating sick sinus syndrome. The traditional Chinese medicine composition is prepared from radix astragali, honey-fried herba ephedrae, radix codonopsis, cornus officinalis, deerhorn glue, psoralea corylifolia, epimedium, tuber of dwarf lilyturf, radix scrophulariae, ligustrum lucidum, wolfberry, ligusticum wallichii, bitter orange, turmeric root tuber, lumbricus, licorice and monkshood. The traditional Chinese medicine composition can be used in clinic treatment of sick sinus syndrome.

The invention relates to a traditional Chinese medicine for treating the sick sinus syndrome and a preparation method thereof. The traditional Chinese medicine is prepared from ephedra, gypsum, semen brassicae, dried ginger, prepared aconite root, white atractylodes rhizome, asarum, fructus aurantii, donkey-hide gelatin, dwarf lilyturf root, Chinese angelica, American ginseng, dried rehmannia root, red ginseng, herba epimedii, fruit of Chinese wolfberry, platycodon grandiflorum, cornus officinalis, astragalus and cinnamon. The preparation method comprises the following steps: firstly, drying, crushing and sieving the gypsum, the semen brassicae, the asarum, the fructus aurantii and the cinnamon to obtain fine powder; then, decocting the ephedra, the dried ginger, the prepared aconite root, the white atractylodes rhizome, the Chinese angelica, the American ginseng, the dried rehmannia root, the red ginseng, the herba epimedii, the fruit of Chinese wolfberry, the platycodon grandiflorum, the cornus officinalis and the astragalus for 0.5-2 hours by virtue of a decocting method, filtering to obtain a filtrate, decocting the filtrate, the donkey-hide gelatin and the fine powder prepared in the process a for 0.5 hour by virtue of the decocting method, and continuously stirring and mixing to form the traditional Chinese medicine. Compared with the prior art, the traditional Chinese medicine has the advantages of reliable curative effect and no toxic and side effects, is simple in production process and remarkable in curative effect for a patient with the sick sinus syndrome.

The invention relates to a traditional Chinese medicine composition for treating sick sinus syndrome. The composition comprises following components: 18-22 g of codonopsis pilosula, 10-14 g of cassia twig, 6-10 g of honey-fried herba ephedrae, 13-17 g of radix paeoniae alba, 8-12 g of radix aconiti lateralis praeparata, 10-14 g of cortex cinnamomi, 13-17 g of longstamen onion bulb, 7-11 g of snakegourd fruit, 13-17 g of milkwort root and 6-10 g of fossil bone.

The invention discloses a traditional Chinese medicine for treating chronic cardiac insufficiency, sick sinus syndrome, ischemic angina pectoris, coronary heart disease and the like, and a preparation method thereof. When the traditional Chinese medicine disclosed by the invention is used for clinical evidence, for deficiency of the heart and kidney-yin and stagnation of heart meridian by differentiation of symptoms and signs of traditional Chinese medicine, the total effective rate for treating congestive heart failure is 88.89%, the total effective rate for treating sick sinus syndrome is 85%, and the total effective rate for treating coronary heart disease caused angina pectoris is 90%. The traditional Chinese medicine is a multi-target good medicine convenient and easy to use for heart, and has conformance to the integral function of the heart. The invention is characterized in that the traditional Chinese medicine is a new medicine which is triggered by symptoms of disease, has a compliance mode of action, is capable of better adjusting the continuous coupling function of the heart, and is favorable to the comprehensive recovery and reconstruction of the integral function of the heart. The medicament disclosed by the invention has the following characteristics of exciting the pacemaker when the pacemaker is weak, strengthening the cardiac efficiency when the myocardial contractive power is weak, improving microcirculation and promoting tissue reconstruction and function recovery when the coronary flow is small, and the like, and the medicament is suitable for all kinds of heart diseases and favorable to comprehensive and integral function recovery of the heart.

The invention provides an oral medicine for treating the sick sinus syndrome and a preparation method of the oral medicine. The medicine comprises components in parts by mass as follows: 1-3 parts of prepared lateral roots of aconite, 1-3 parts of dried ginger, 1-3 parts of radix glycyrrhizae preparata, 1-3 parts of Chinese angelica, 1-3 parts of red paeony roots, 1-3 parts of szechuan lovage rhizome, 1-3 parts of safflower, 1-3 parts of danshen roots, 1-3 parts of ladybell root, 1-3 parts of astragalus membranaceus, 1-3 parts of epimedium herb and 1-3 parts of manchurian wildginger. The preparation method of the oral medicine comprises steps as follows: high-quality and first-grade prepared lateral roots of aconite, dried ginger, radix glycyrrhizae preparata, Chinese angelica, red paeony roots, szechuan lovage rhizome, safflower, danshen roots, ladybell root, astragalus membranaceus, epimedium herb and manchurian wildginger are purchased in parts by mass and ground to granules for standby; the ground granular medicines are put into a cloth bag, an opening of the cloth bag is tied, then the cloth bag is soaked in cooled boiled water for about half an hour until medicine tissue is fully soaked, then the well soaked medicines and a supernatant liquid for soaking the medicines are put into a medicine decocting machine together, cooled boiled water is added until the water level is 35 mm higher than the medicines, the medicines are decocted twice, the medicines are decocted for 30 minutes for the first time, after a medicine liquid is filtered, then medium-temperature boiled water is added until the water level is 30 mm higher than the medicines, the medicines are decocted for 25 minutes for the second time, and the medicine liquids obtained after two times of decoction are mixed and condensed to form 1200 mm of a 50% traditional Chinese medicine liquid.

A method for detecting heart rhythm irregularities, including the steps of: measuring pulse peaks continuously over a period of time to obtain pulse peak sequence and pulse peak time interval sequence corresponding to the pulse peak sequence; and calculating the absolute value of a difference between a single pulse peak time interval in the pulse peak time interval sequence and an average value of the pulse peak time interval sequence, and determining that an irregular pulse peak (IPP) has occurred if the absolute value is larger than or equal to 15% to 25% of the average value of the pulse peak time interval sequence. A detection device using the method is also provided. This uses a non-invasive approach to detect heart rhythm irregularities such as sick sinus syndrome, irregular pulse peaks, irregular heartbeat, and atrial fibrillation, and can be applied to a sphygmomanometer or a wearable device.

The invention relates to a meglumine adenosine cyclophosphate powder injection medicine composition for injection and a preparation method thereof. Specifically, the invention relates to a medicine composition on one hand and the medicine composition comprises adenosine cyclophosphate, meglumine, mannitol and an optional acid-alkali regulator, wherein based on every 10 parts of adenosine cyclophosphate by weight, the medicine composition comprises the following materials in parts by weight: 5.5-6.5 parts of meglumine, 9-15 parts of mannitol and a proper use amount of the acid-alkali regulator; and the use amount of the acid-alkali regulator ensures that when the medicine composition is dissolved and diluted in water to prepare a solution every 1ml of which contains 10mg of adenosine cyclophosphate, the pH value of the solution is 5.5-7.0. The powder injection composition disclosed by the invention can be used for treating cardiac failure, myocarditis, sick sinus syndromes, coronary heart diseases and cardiomyopathy and can also be applied to adjuvant therapy of cardiac arrhythmia; and the meglumine adenosine cyclophosphate powder injection medicine composition for injection disclosed by the invention has good pharmaceutical properties.

The invention relates a medicine for treating the sick sinus syndrome.The medicine is prepared from, by weight, 12 parts of fructus rosae laevigatae, 5 parts of herba asari, 9 parts of radix polygonati officinalis, 12 parts of platanthera japonica (Thunb.) Lindl., 12 parts of ilex aculeolata Nakai, 12 parts of colysis bonii Ching and 3 parts of wikstroemia pilesa Cheng.In application of the medicine, fructus rosae laevigatae has the effects of securing essence and reducing urination, and astringing intestines and checking vaginal discharge; herba asari has the effects of expelling cold and wind, relieving pain, warming the lung to reduce watery phlegm, and opening orifices; radix polygonati officinalis has the effects of nourishing yin and moistening the lung, and nourishing the stomach to improve the production of body fluid; platanthera japonica (Thunb.) Lindl.has the effects of tonifying qi and moistening the lung, eliminating phlegm and stopping coughs, and clearing away toxic materials; ilex aculeolata Nakai has the effects of dispelling wind and reducing phlegm, and clearing away heat and toxic materials; colysis bonii Ching has the effects of promoting blood circulation to remove blood stasis, and reducing fever and causing diuresis; wikstroemia pilesa Cheng has the effects of expelling water retention and diminishing swelling, and clearing away toxic materials and removing stasis.All the medicinal materials are used in cooperation, and the effects of tonifying qi and nourishing yin, nourishing the heart and dredging collaterals, warming and smoothing the heart and the kidneys, and tonifying qi and restoring the pulse are achieved.Clinical experiments prove that the medicine can safely and effectively treat the sick sinus syndrome.

The invention relates to a Chinese medicine composition for curing the sick sinus syndrome and belongs to the field of traditional Chinese medicine. The Chinese medicine composition is characterized by comprising the following Chinese medicine raw materials in weight part: 15 parts of Radix Astragali, 6 parts of ginseng, 6 parts of Chinese garlic, 5 parts of Asarum Herb, 5 parts of Chinese cassia tree, 6 parts of dried orange peel, 9 parts of bighead atractylodes rhizome, 6 parts of pinellia ternate, 15 parts of trichosanthes kirilowii maxim, 6 parts of medicated leaven, 10 parts of chicken's gizzard membrane and 6 parts of honey-fried licorice root. The Chinese medicine composition has the efficacies of freeing the yang, restoring the pulse, resolving the depression, activating the spleen, reducing the phlegm and repelling the moisture. In the aspect of curing the sick sinus syndrome, the Chinese medicine composition is obvious and stable in curative effect, and free of toxic / side effects.

The invention relates to the fields of biomedical engineering technology and regenerative medicine and particularly relates to a method for constructing a tissue-engineered pulsating tissue. According to the method disclosed by the invention, stem cells derived from brown adipose are induced into sinuatrial-node-like cells, and the sinuatrial-node-like cells are implanted onto collagen sponge to construct the tissue-engineered sinuatrial-node-like pulsating tissue in vitro. The tissue-engineered tissue with pulsating characteristics obtained by virtue of the method provides a tissue model for a research on screening of arrhythmic drugs and brings hope for treatment of arrhythmic diseases caused by sick sinus syndrome and auriculo-ventricular block.