The invention discloses an interconnection test fixture and an interconnection test method for a silicon-based component, and belongs to the technical field of advanced electronic packaging, the interconnection test fixture for the silicon-based component comprises a vacuum adsorption hole, an X-axis limiting baffle block, a Y-axis limiting baffle block and a test area Z plane regulator, and realizes three-dimensional regulation of a tested piece. The problems of clamping and compatibility of different silicon-based assemblies are solved, the fragment risk is avoided, and the purposes that the test fixture is universal and convenient to use are achieved; according to the interconnection test method, direct current and alternating current characteristic tests are carried out by using an isolation technology, and test errors are reduced by reducing generated current branches, so that the accuracy of a measured value is ensured; according to the invention, the testing of the microsystem component level is realized, most of the fault components can be detected before the function performance test, the final function performance test first pass yield is improved, the subsequent potential quality hazard and resource waste are avoided, and the test coverage rate of the network is effectively improved.