The present application relates to the technical field of
plant science, and particularly relates to a device and method for measuring the anatomical structure of
plant fine roots without thin sectioning. By directly
staining, stably positioning and microscopically observing the cross section of fine roots, clear and complete anatomical images of the cross section of fine roots can be obtained without
dehydration, embedding and ultrathin sectioning, and reliable measurement of anatomical parameters can be realized. The steps of fixing, dehydrating, embedding and ultrathin sectioning are omitted, the
sample preparation process is significantly simplified, the time from
processing to imaging is shortened, the overall measurement efficiency is improved, and the device is suitable for multi-sample and batch analysis scenarios. Under the premise of ensuring the reliability of the identifiable and measurable anatomical structure, the process is simplified, the efficiency is improved and the cost is reduced, and the device has good technical value and application prospect.