The invention relates to the field of canal treatment and specifically relates to a canal water pollution superior microorganism in-situ ecological restoration method. The canal water pollution superior microorganism in-situ ecological restoration method comprises the steps of measuring parameters of a canal water environment, acquiring and detecting a water sample, acquiring a bottom mud of a canal, preparing a microorganism domestication primary liquid, preparing an enrichment culture medium, domesticating the microorganism, preparing a solid inoculant, putting the solid inoculant into the canal, and detecting whether the parameters of the water sample reach the standards within 10-30 days and 80-90 days starting from the time when the solid inoculant is put. According to the method, the construction and operation costs are low, the construction is convenient and environment-friendly, and does not affect the surrounding resident and environments, the management and the maintenance are simple, no secondary pollution is caused, the purification and restoration effects are good, the self-cleaning capacity of a canal water body is strengthened after the treatment for half a year to one year, local grasses and creatures naturally grow and propagate, an original biologic chain is restored, and the purpose of in-situ biological restoration of the water environment is fulfilled.