The invention discloses a descending movable framework for a highway box girder at a highway and railway combined construction section and an application method of the descending movable framework and relates to the field of bridge construction. The descending movable framework comprises bearing girders, guide beams, support devices, lifting support frames, a template system, a bottom die opening and closing system and a longitudinal shift system. When the descending movable framework for the highway box girder at the highway and railway combined construction section is at the state of concrete pouring, the lifting support frames are abutted to the lower end faces of the bearing girders, at the middle positions of every two pier bodies, the pouring load of the highway box girder is transmitted to other structures, and the lifting support frames are composed of distribution beams, upper sleeve rods, pin rolls, lower sleeve rods, sleeper beams, platforms, sand cylinders, jacks, rigid pads, walking wheels and steel rails. According to the descending movable framework and the application method, the additionally arranged lifting support frames with liftable height can effectively solve the technical problems of stress and hole passing of the framework.