The invention relates to the technical field of hydro-optical needles, in particular to a sixteen-hole hydro-optical needle which comprises a shell, an injection mechanism is arranged at the bottom of the shell and comprises a mounting plate, a hydro-optical needle head, an external thread sleeve, a thread groove, a sealing ring, a groove and a rotating ring, and a liquid storage mechanism is arranged in the shell and above the injection mechanism. The liquid storage mechanism comprises a push piston, a push rod, a servo motor, a baffle plate, a liquid outlet hole, a through hole, a liquid injection hole, a sealing plug and a reset spring, by arranging the shell, the injection mechanism and the liquid storage mechanism, the problem that a single-needle hydro-optical needle is replaced by a multi-needle hydro-optical needle commonly used in the market at present is solved, and the multi-needle hydro-optical needle structurally comprises a shell, a needle seat and a plurality of needle heads; the problems that a plurality of needle heads are installed on a needle base, a patient needs to inject injections for multiple times, the needle heads need to be replaced after each injection is injected, the needle heads need to be taken down one by one and then new needle heads need to be installed during replacement, and operation is inconvenient are solved.