The invention discloses a meat online non-destructive testing method and apparatus based on fusion of image and spectrum information, including a conveyor belt, a spectrum collection room, an image collection room, a camera mounted beam, an imaging source, a camera, a Y type optical fiber, a spectrograph, a data processing PC, two photosensors, a motor and a conveyor belt bracket. Through online collection of meat image and spectrum information, collecting feature information of characteristic meat quality, establishing a fused quantification prediction model based on image characteristics, spectrum characteristics and characteristics layer data of image and spectrum, using the obtained quantification prediction model to combine with quality evaluation system to establish a decision layer data fusion model based on image and spectrum for testing meat online. The invention uses complementarity and redundancy of the image information and the spectrum information to implement classification of materials and quality test during meat producing process according to optical feature of meat, which improving production efficiency.