The invention discloses a micro-vessel liver
chip based on
cell clusters. The micro-vessel liver
chip comprises a micro-vessel
system located on the upper layer, a blood-vessel-
endothelium-like barrier
system in the middle and a liver-organ multi-
cell co-culture
system on the lower layer, wherein the micro-vessel system and the liver-organ multi-
cell co-culture system are arranged on own substrates respectively. The micro-vessel system comprises a bent vessel composed of multiple flow-stopping barriers in a staggered mode, and a micro-vessel inlet and a micro-vessel outlet are formed in the two ends of the bent vessel respectively. The blood-vessel-
endothelium-like barrier system is composed of a
porous film. The liver-organ multi-cell co-culture system comprises a
cell cluster enrichment region and a multi-cell co-culture region, and a culture system inlet and a culture system outlet are formed in the two ends of the liver-organ multi-cell co-culture system respectively. A
liver disease model can be established, research on
pharmacokinetics and
medicine activity can be performed, and the micro-vessel liver
chip has the advantages of using a small quantity of samples, achieving
medicine low consumption and being portable, economical, efficient and accurate.