The invention discloses a multi-layer annular bicycle stereo garage and an operation method. The multi-layer annular bicycle stereo garage comprises a garage rack, a protection mechanism, a vertical transmission mechanism, a bicycle fixing frame and horizontal transmission mechanisms; the garage rack is perpendicularly arranged on the ground, the top of the garage rack is a parking area, and the protection mechanism is arranged on the outer side of the parking area; and the vertical transmission mechanism is arranged in the axial direction of the garage rack, and the parking area is provided with a plurality of layers of horizontal transmission mechanisms. Different from an existing parking garage, the advantage that bicycles are light is utilized, grooves of bicycle frames incline centripetally towards the center of the annular garage, centripetal force is provided for bicycles, due to the fact that the circular motion speed is small, centrifugal force is not large, meanwhile baffle plates are designed on rear wheels of a bicycle frame structure, the bicycles can be prevented from moving at will, by means of the design, a bicycle clamping device is not needed for clamping wheels,the bicycle frame structure is simplified, the compatibility of bicycle types is improved, and the manufacturing cost is saved.