The invention belongs to the field of plant cultivation, and discloses a seedling cultivation pipeline assembly for seedling automated circulation and manual fixed point operation. A factory area is divided into areas with different temperature and humidity environments, including a material storage area, a crop processing area, a crop growth promoting area, a crop growth area, a washing area, anda finished product storage area, wherein the crop growth promoting area is divided into a germination area and a healing area; seedbeds and/or trays are adopted as basic logistics units, and the pipeline assembly for circulating seedlings in the above areas is composed of wheeled rails, push-pull rails, conveyor belts, light-duty cranes, and mobile lifts. The invention also provides the using method of the seedling cultivation pipeline assembly for seedling automated circulation and manual fixed point operation. According to the seedling cultivation pipeline assembly for seedling automated circulation and manual fixed point operation and the using method thereof, a continuous logistics line is adopted for connecting working points, and a production mode in which the crops to be operated are transferred through the automated logistics line and operators operate at fixed points is achieved. The operators' daily walking distance is reduced by 90%, and employee's labor intensity is reduced by 30%.