The invention discloses a closed-type constant-temperature multi-layer water circulation turtle cultivation system which comprises a multilayer turtle cultivation pool devices, a cultivation water environment adjusting device, an indoor air environment adjusting device and a cultivation system operation control device, wherein the multilayer turtle cultivation pool device comprises a plurality of turtle cultivation pools, circulating water return pipes, circulating water outlet branch pipes and main circulating water outlet pipes, primary solid liquid precipitation devices, blow-down valves and pollution discharge blow-down pipes; the cultivation water environment adjusting device comprises a solid-liquid separator, a blow-down valve, a sterilizer, a protein separator, a biological filtering device or tank, a circulating pump and a water heating device; and the indoor air environment adjusting device comprises an air heating device, an air oxygenating device and an insulation building. According to the closed type constant-temperature multi-layer water circulation turtle cultivation system, turtle cultivation environments can be comprehensively controlled, the land, the water and the energy are saved, an optimal ecological environment suitable for turtles to rapidly grow is established, turtle cultivation is free of drugs, and healthy and safe food sanitation requirements are met.