The invention relates to the field of medical apparatus and instruments, and in particular relates to a disinfection device for medical apparatus and instruments. The disinfection device comprises a disinfection box, a layer partition plate, a bottom plate, round rolls and a conveying mechanism, wherein the layer partition plate and the bottom plate separate the disinfection box into a part feed chamber, a disinfection chamber and a mounting chamber, the round rolls are fixed to the side wall of the disinfection chamber, and a sliding belt sleeves the round rolls; a plurality of part containing boxes with grids are fixed on the sliding belt; grid-shaped box covers are hinged to the part containing boxes, and fasteners matched with the part containing boxes are arranged on the box covers; an air cylinder is fixed on the inner wall of the disinfection chamber, a flapping rod is hinged to the inner wall of the disinfection chamber, and the free end of an output shaft of the air cylinder is hinged to the flapping rod; the conveying mechanism is positioned in the part feed chamber, the conveying mechanism comprises a driving wheel and a driven wheel, a pressure sensor is mounted in thedriven wheel, and the pressure sensor is serially connected with the air cylinder through a controller. With the adoption of the technical scheme, the disinfection device has the advantages that the medical apparatus and instruments can be circularly taken, and the problem that some medical apparatus and instruments are positioned at the bottom of the disinfection chamber for a long term so as tobecome rusty is solved.