5 results about "Glycan biosynthesis" patented technology

This invention belongs to the field of genetic engineering technology, specifically relating to the application of OpgG and its mutants in the production of human milk oligosaccharides. This invention provides a mutant of the dextran biosynthetic protein OpgG, which is obtained by mutating the 443rd amino acid from threonine to proline based on the wild-type dextran biosynthetic protein OpgG shown in SEQ ID NO.1. This mutant is introduced into human milk oligosaccharide-producing strains. opgG The gene, and / or the T443P mutant gene, was applied to the production of human milk oligosaccharides, resulting in a significant increase in the yield of 3'-SL, 6'-SL, LNT II, ​​LNnT, and LNT.

Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a variant ABC transporter protein YOR1 from Saccharomyces cerevisiae with improved 2′-fucosyllactose export activity and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.

Compositions, foodstuff and methods are provide herein from cereal plant or a part thereof, wherein the plant or part thereof has an altered (I,3;1,4)-p-glucan content as compared to a wild-type cereal plant or part thereof, wherein said, plant or part thereof carries one or more mutations in the CslF6 gene, wherein said mutated. CslF6 gene encodes a mutant CslF6 polypeptide, wherein said mutant CslF6 comprises at least one substitution, addition or deletion of an amino acid in a switch motif of CslF6, wherein the switch motif comprises SEQ ID NO: 14.

This invention provides a filamentous fungus in which wall growth formation is suppressed, and the use of said filamentous fungus. [Solution] Use a filamentous fungus lacking one or more genes selected from the galactosaminogalactan biosynthesis gene group and one or more genes selected from the chitin biosynthesis gene group, or a filamentous fungus in which the expression of one or more genes selected from the galactosaminogalactan biosynthesis gene group and one or more genes selected from the chitin biosynthesis gene group is suppressed.

The invention discloses serine type carboxypeptidase for inhibiting growth of blue-green algae and belongs to the technical field of biology and environmental protection, bacillus altitudinis FY1 is screened and separated from a rice field water sample, it is found that protein serine type carboxypeptidase exists in the FY1 in the peptidoglycan biosynthesis process, the nucleotide sequence of the protein serine type carboxypeptidase is shown as SEQ ID 0.1 in a sequence table, and the nucleotide sequence of the protein serine type carboxypeptidase is shown as SEQ ID NO. The serine type carboxypeptidase is targeted to cell walls of cyanobacteria, has an efficient splitting effect on the cyanobacteria, can kill harmful cyanobacteria and is used for treating cyanobacteria bloom.