The invention discloses a surface plasmon imaging system based on differential denoising. The surface plasmon imaging system comprises a rotary illumination system, a tube mirror, a beam splitter, an oil immersion microscope objective, a gold film substrate, a high-precision displacement platform and an imaging light path, and a surface plasmon has the characteristics of local field enhancement and sensitivity to environment refractive index change, and can be used for imaging small particles. The system comprises the following steps: firstly, performing rotary illumination on an object by using incident light by using a rotary illumination system, eliminating interference fringes generated by the incident light and scattered light, and obtaining an image without the interference fringes and with a smaller size; and on the basis, using a differential denoising method to quickly move a high-precision displacement platform, so that the background noise of the image is removed, and the signal-to-noise ratio of imaging is improved. According to the imaging system, the signal-to-noise ratio of surface plasmon microimaging is greatly improved by using a differential denoising method, high-contrast imaging can be carried out on nanoparticles with the diameter as low as 22 nm, and the particle size resolution is 10 nm.