The invention discloses a catheter twining preventive comprehensive therapy apparatus, comprising a base, a tank connected to the top of the base, a negative pressure aspiration system positioned on the tank, and an ozone therapy system; the negative pressure aspiration system comprises an electromagnetic negative pressure pump, a negative pressure meter, a one-way valve, a drainage bottle and a negative pressure therapy port that are connected in sequence; a liquid bottle on the ozone therapy system is further connected with a collection bottle, an ultraviolet irradiator is disposed at the top of the tank, a line of a positive pressure connecting tube and a negative pressure connecting tube is also provided with a plurality of limit fasteners, the side of the tank is also provided with an adjusting wheel, and the positive pressure tube is locked to the adjusting wheel. Through the above structure, it is possible for ozone liquid to carry high-frequency oscillatory energy, and it is possible to better provide effective separation and cleaning for wound deposition, blood stain, necrotic issues and secretion; the lines on the therapy apparatus in the scheme rarely twine together, subsequent separation is facilitated, and maintenance efficiency for maintainers and safety coefficient of use are increased.