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Method and apparatus for identifying classifying or quantifying DNA sequences in a sample without sequencing

InactiveUS6141657ARapid and economical and quantitative and precise determination and classificationSufficient discrimination and resolutionData processing applicationsDigital data processing detailsSequence databaseDNA Sequence Databases
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in question and to carefully choose target subsequences in order that experiments yield a maximum amount of information.
Owner:CURAGEN CORP

Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing

InactiveUS6418382B2Rapid and economical and quantitative and precise determination and classificationSufficient discrimination and resolutionData processing applicationsMicrobiological testing/measurementSample sequenceSingle sequence
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in question and to carefully choose target subsequences in order that experiments yield a maximum amount of information.
Owner:CURAGEN CORP

Safety air bag inflation device

An air bag inflator providing a source of gas, releasable upon command, to inflate a supplemental inflation restraint (SIR) system commonly known as an automobile air bag. Provided is a pressure vessel containing one or more separate chambers for the purpose of storing gaseous fuel(s) and gaseous oxidizer(s) or liquid fuel(s) and liquid oxidizer(s) under pressure with helium as the primary filler gas. The primary function of the helium gas is to serve as a kinetic damper to modulate and control the reaction rate of the fuel(s) and oxidizer(s). Because of its low mass, high thermal conductivity and high heat capacity for its mass, helium is an excellent filler gas. In the case of the gaseous fuel(s) and oxidizer(s) a single chamber is provided. In the case of liquid fuel(s) and oxidizer(s), two or more separate housings are provided for storing the liquid fuel(s) and oxidizer(s). Along with the liquid fuel(s) and oxidizer(s) housings, two separate chambers containing pressurized helium are provided within the pressure vessel. The first helium chamber rapidly pressurizes upon initiation of a gas producing pyrotechnic igniter. This pressure acts on thin membranes on the first chamber side of the separate liquid storage housings to force the liquid fuel(s) and oxidizer(s) into the second chamber where the materials are atomized and mixed. The mixture is ignited by the arrival of hot gases from the igniter directed into the second chamber via the small diameter tube or orifice. The fuel(s) oxidizer(s) mixture burns to produce gaseous reaction products that are released into the air bag by bursting a controlled rupture burst disc.
Owner:AUTOLIV DEV AB

Method and apparatus for identifying, classifying, or quantifying protein sequences in a sample without sequencing

InactiveUS6432361B1Rapid and economical and quantitative and precise determination and classificationSufficient discrimination and resolutionData processing applicationsMicrobiological testing/measurementSequence databaseDNA Sequence Databases
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in question and to carefully choose target subsequences in order that experiments yield a maximum amount of information.
Owner:CURAGEN CORP
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