The invention provides a premix mate for laying hens in an egg producing period and a preparation and application of the premix mate and belongs to the field of animal feeds. The premix mate for the laying hens in the egg producing period is prepared from the following raw materials in parts by weight: peanut meal, hot chili residues, fermented cake meal and amino acid, sodium bicarbonate, betaine, hemoprotein, limestone powder, mycoprotein, a shell treasure, calcium hydrophosphate, quercetin, a mildew inhibitor, perennial sowthistle powder, compound probiotics and the like. According to the premix mate for the laying hens in the egg producing period, one part of bean pulp in a complete formula feed can be replaced, the antioxidant activity of the laying hen bodies can be effectively improved, the digestion and absorption rate and the conversion rate of the laying hens in the egg producing period are effectively improved, the immunity is significantly improved, healthy intestinal tracts of the laying hens are ensured, too thin faeces are prevented, unhairing of the egg producing period is improved and slow growth condition of feather is improved. For the conditions of poor quality of egg shells, multiple broken eggs and a short peak laying period, the colors and the quality of the egg shells are effectively improved, the health of bodies is promoted, the production performance is improved and the egg production period is prolonged.