The invention relates to a preparing method for a unidirectional wet-conduction pure cotton fabric based on nano-zinc oxide. The preparing method includes the steps that (1) nanometer ZnO powder and aphthalic-ester coupling agent are mixed, then Tween-80 is added, dispersion is conducted, and modified nanometer ZnO finishing liquid is obtained; (2) the cotton fabric is soaked into the modified nanometer ZnO finishing liquid, two times of soaking and two times of rolling are conducted, then pre-baking and baking are conducted, and the nanometer-ZnO-finished cotton fabric is obtained; (3) printing net is put on the nanometer-ZnO-finished cotton fabric, slurry is put, slurry is scraped at constant speed, then the fabric is pre-baked, baked, soaped and dried, and the unidirectional wet-conduction pure cotton fabric is obtained. According to the preparing method for the unidirectional wet-conduction pure cotton fabric based on the nano-zinc oxide, through compound function finishing, the fabric has the excellent moisture absorption and nice and cool effect, and the thermal-wet comfort requirement when people are in hot weather and strenuously exercise is met.