The invention relates to a novel ICF (initial confinement fusion) pellet detection device and a positioning method. The device comprises a laser, a beam expander, a first bean splitter, a second beamsplitter, a third beam splitter, a plane mirror, a to-be-detected pellet, a lens, a CCD image sensor, a computer, a high-power LED and a diaphragm. The pellet is moved at the internal of 10 mu m, a backlight projection image in each position is collected, image preprocessing work such as filtering and aperture extraction is performed, the acutance of an area near a bright ring is calculated, and the position with the largest acutance meets the object-image conjugate relation. The pellet is moved in the interval of 10 mu m at the interval of 1 mu m continuously after being moved to the position. The steps of collection, image preprocessing and acutance calculation are repeated, the pellet is moved to the new position with the largest acutance, and positioning is finished. The novel pellet detection device and the positioning method are applicable to conventional cylindrical vacuum space and can realize automatic positioning quickly and accurately.