The invention discloses a manufacturing method of a high-opening SE net-knot-free screen printing plate. The manufacturing method comprises the following steps of piece cutting, net aligning, film laminating, pressing, net opening, scanning, laser removing, coating, film layer laser removing, alignment exposure, inspection and shipment. According to the method, the gauze in an effective area of apattern is removed at a fixed point by using laser, so that the film raw material cost is greatly saved, the overall platemaking time is saved, and the platemaking efficiency is improved; meanwhile, the gauze in the corresponding area is removed through lasers, poor screen printing plates and raw material scrapping caused by the screen stretching process are reduced, the production cost of a company is reduced, meanwhile, a scanning program and a laser removing program run synchronously, and the existing platemaking working hours are reduced; and the gauze in the area corresponding to the pattern is removed by laser without changing the pattern of a client, so that a net-knot-free screen printing plate required by SE technology printing can be manufactured.