The invention provides an anti-seepage water stop structure on a water conservancy project, and relates to the field of water conservancy projects. The anti-seepage water stop structure comprises a concrete layer, wherein a waterproof plate is fixedly installed on the bottom side of the concrete layer, a same water tank is fixedly installed on one side of the concrete layer and one side of the waterproof plate, a material box is arranged in the water tank, an inserting hole is formed in the water tank, a filter box is inserted into the inserting hole, the bottom side of the filter box is in contact with the material box, and a flow guide plate is fixedly installed in the filter box. According to the anti-seepage water stop structure, a pull rod and a cleaning structure assembly are arranged, so that a cleaning rod cleans the top side of a filter plate through a plurality of brushes, and then the smoothness of the filter plate is guaranteed; meanwhile, a water stop plate is vertically arranged, so that water outlet is facilitated; and in addition, the filter box and a cover plate are arranged, when a large amount of silt is accumulated in the filter box, the filter box can be pulledout by pulling the cover plate, then two bolts are disassembled, then the cover plate can be taken down, so that the silt in the filter box can be conveniently cleaned, and the use of the filter boxis more convenient.