The invention provides a zero leakage inserting installation one-way valve which comprises a valve element, a valve seat, a spring, a compression nut, sealing rings and stopping rings. A guiding shaft of the valve element is arranged in the valve seat, one end of the spring is arranged on a location shaft of the valve element in a sleeved mode, the other end of the spring is arranged in a location hole of the compression nut in a sleeved mode, the compression nut is connected with the valve seat, the first stopping ring and the first sealing ring are arranged in a sealing groove of the compression nut to be sealed, the second stopping ring and the second sealing ring are arranged in a sealing groove of the valve seat, a V-type groove structure is formed between the valve element and the valve seat, and an O-type sealing ring is arranged in a V-type groove. According to the zero leakage inserting installation one-way valve, a sealing structure mode that the V-type groove is combined with the O-type sealing ring is adopted, the reverse sealing performance is good, and the zero leakage requirement can be met. Meanwhile, the zero leakage inserting installation one-way valve has the advantages of being low in manufacturing cost, reliable in working performance, high in reliability, small in size, light in weight, compact in structure, convenient to operate, easy to disassemble and assemble and the like, and the zero leakage inserting installation one-way valve is suitable for systems with compact space structures.