The invention relates to a method for cultivating nucleus pearls, and belongs to the technical field of pearl cultivation. The method comprises the steps as follows: step one, taking out a pearl nucleus to be put into a first treating fluid for soaking and cultivation; step two, taking out a cell chip of the edge membrane of the rear half part of a mussel body to be put into a second treating fluid for soaking and cultivation; step three, enabling the cell chip to be adhered to the pearl nucleus and planted into the mussel for cultivation so as to form a nucleus pearl, wherein the first treating fluid comprises the components as follows: a bamboo charcoal powder solution, a dry chrysanthemum solution, a gold mudstone solution, a gobi stone solution, a safflower parasitic skin solution, a peach parasitic skin solution, a safflower loranthus parasiticus skin solution and a vitamin B solution; the second treating fluid comprises the components as follows: acid red, chondroitin, mafenide, taurine, sodium chloride, hyriopsis cumingii polysacchride, vitronectin and water. According to the method provided by the invention, the rejection of the mussel body to pearl nucleus is overcome, the vitality of the cell chip is promoted, and large-scale operation can be implemented.