Disposable microfluidic cartridges, potentiostat reader devices, systems, kits, and methods to determine the concentration of analyte reaction products in a patient's specimen, such as: blood, saliva, cerebrospinal fluid, joint fluid or tears. The microfluidic cartridges are disposable, paper-based strips comprising multiple assay layers, each layer comprising: a drop zone for receiving a patient specimen; a filter to remove contaminates, and / or isolate assay reactants; a hydrophilic microfluidic channel to direct movement of the specimen down the cartridge; a centered reaction chamber comprising impregnated enzymes and reagents to chemically react with the filtered specimen to produce an analyte; and a detector mechanism. Multiple analytes can be produced in parallel (e.g. one per layer), or sequentially along one layer. The detector mechanism utilizes electro-analytic methods to facilitate the reader device in quantifying each analyte, and each analyte concentration is wirelessly transmitted to an electronic computing device for storage in a patient's medical record.