53 results about "G1 phase" patented technology

The present invention relates to the downregulation of surface receptor CCR5 expression through manipulation of the cell cycle in activated lymphocytes by administering a composition that arrests the G1 phase of the cell cycle, thereby reducing receptor sites for entry of HIV into T cells, and thus, the effects of HIV. Further, compositions are disclosed that include at least one G1 phase arresting agent and at least one antiviral agent, wherein the combination of agents synergistically enhances the activity of the antiviral agent.

The present invention refers to steroid derivatives for use as medicaments. More specifically, the invention also relates to the use of a steroid derivative of 5-androstene-, 5-pregnenolone or corresponding saturated derivatives (androstane- or pregnane-) in the manufacture of a medicament for the treatment of a benign and / or malignant tumour, which medicament is capable of interrupting disturbances in Wut-signaling, such as cell-cycle arrest in G1-phase, and / or providing an angiostatic effect. Examples of such steroid derivatives are -5-androstene-17-ol, androstane-17-ol-pregnane-17-ol or pregnane-17-ol derivatives. In a further aspect, the invention relates to a method of producing a medicament for the treatment of a benign and / or malignant tumour and / or an inflammatory condition comprising the steps of contacting 5-androstane-3B,17-dio 1 or androstane-3B-diol, an enzyme and a sulfotransferase to provide 5-androstene-17-ol-3B-sulfate or corresponding andros tane derivative (17-AEDS or 17-AADS); and mixing the 17-AEDS or 17-AADS so produced with a suitable carrier; whereby a medicament which is capable of acting as a ligand to peroxisome proliferators-activated receptor—(PPAR) is produced.

The invention relates to an miR-21 small molecule inhibitor and an application The medicament of the invention can specifically inhibiting the expression of miR-21, thereby promoting a compound 2,4-diamino-1,3-diazacyclohexane-5-nitrile which can induce various tumor cell apoptosis. The binding capability of 2,4-diamino-1,3-diazacyclohexane-5-nitrile is that: Ki=1.49nM, deltaG=-12.04kcal / mol. The preparation method comprises: dissolving 2,4-diamino-1,3-diazacyclohexane-5-nitrile in deionized water to prepare mother solution, adding the mother solution into a tumor cell (colloid tumor, stomach cancer and breast cancer) culturing system according to a certain proportion. The medicament can specifically inhibit the expression of miR-21 without affecting the expression of miRNA. The cell cycle appearances G0 / G1 phase arrest, the cell apoptosis proportion is obviously increased, and more effective inhibition to tumour can be implemented.

A method of nuclear transfer is described which comprises transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion.

The invention provides a method for promoting secretory expression of rhIL-24 by an engineering cell strain by using sodium butyrate, belonging to the technical field of gene expression. On the basisthat a site-specific integrated engineering cell strain FCHO / IL-24 for secretory expression of the rhIL-24 is subjected to adherent culture with a culture medium containing 10% of serum, adherent culture with a culture medium containing 0.5% of serum adherence and suspension culture with a culture medium containing 0.5% of serum, the cell strain is treated by using sodium butyrate with different final concentrations (0, 0.125, 0.25, 0.5, 1 and 2 mmol / L). According to the invention, NaBu can improve the secretory expression level of foreign protein by CHO cells, promotes the secretory expression level of rhIL-24 by engineering cells, promotes generation of G0 / G1 phase arrest of the engineering cells, and can change the metabolic activity of the cells under adherent culture conditions; and by using of the NaBu as an additive of a serum-free culture medium of the cell strain, the secretory expression level of the rhIL-24 by the cell strain is improved in high-density suspension culture, so experimental data is provided for large-scale culture of FCHO / IL-24 cells, and a reference can be provided for expressing gene engineering proteins by using mammalian cells at the same time.

The invention discloses a human PKMYT1AR gene. The nucleotide sequence of the human PKMYT1AR gene is as shown in SEQ ID NO: 1; the reagent for detecting the expression quantity of the human PKMYT1AR gene is applied to preparation of the non-small cell lung cancer clinical diagnosis reagent, the expression level of the human PKMYT1AR gene is in negative correlation with clinical prognosis of the non-small cell lung cancer, and experimental results show that the expression of the human PKMYT1AR gene in a non-small cell lung cancer cell line is higher than that of normal pulmonary epithelial cells; after the PKMYT1AR gene is knocked down, the proliferation of a non-small cell lung cancer cell line is obviously inhibited, and the cell cycle is retarded in a G0 / G1 phase; the killing effect of clinical platinum chemotherapeutic drugs for non-small cell lung cancer on tumor cells can be obviously improved; the invention reveals that the PKMYT1AR gene is a potential risk gene of the non-small cell lung cancer, and PKMYT1AR expression inhibition is combined with a radiotherapy or chemotherapy drug DDP for use, so that the clinical treatment effect of the non-small cell lung cancer is enhanced.

The invention discloses an application of histone methylation H3K4me3 in pig ovary granulosa cells, and belongs to the technical fields of cell engineering and genetic engineering. The invention takes H3K4me3 as an entry point, and uses cell biology methods to study its influence on the function of porcine ovary granulosa cells. The technical scheme of the invention is carefully designed and the result is reliable. In order to confirm the influence of H3K4me3 on the function of porcine ovary granulosa cells, the present invention verifies from multi-level and multi-angle, at cell level and protein level. The present invention clarifies the influence of H3K4me3 on the function of porcine ovarian granulosa cells: H3K4me3 can promote the proliferation of porcine ovarian granulosa cells, inhibit the apoptosis of porcine ovarian granulosa cells, and reduce the proportion of porcine ovarian granulosa cells blocked in the GO / G1 phase; It is of great application value to study the mechanism of histone methylation on the development of ovarian follicles and the reproductive performance of sows.