The invention provides a liquefaction process of glucose syrup. The liquefaction process mainly comprises the following steps: milling, size mixing, gelatinizing, sterilizing and filtering for separating, wherein the milling step comprises directly milling dry raw grains; the size mixing step comprises: firstly stirring and mixing warm water with fine grain powder for size mixing, soaking and stirring for 2-3 hours at a constant temperature condition, and adding a high temperature resistant liquefied enzyme preparation and a protein coagulant. The invention further provides liquefaction equipment of glucose syrup. The equipment comprises a primary flour mill, a primary classifying screen, a soaking tank, a jet tank for gelatinizing, at least two laminar flow tanks, a jet tank for sterilizing, a feed liquid temporary storage tank and a press filter which are serially connected in sequence. Therefore, the liquefaction process and liquefaction device of glucose syrup provided by the invention have the characteristics of simple process, high product extraction rate, reduced raw material waste, low cost, zero emission of wastewater and the like.