The invention discloses a glass steel pipeline on-site repair assembly and method. The assembly comprises a connection structure, a repair pup joint and a conversion pup joint. One end of the conversion pup joint is connected with the connection structure, the other end of the conversion pup joint is connected with the repair pup joint, the repair pup joint is connected with a glass steel pipelineto be repaired, the connection structure comprises a connection sleeve, a connection pup joint, an inner core pipe and an outer core pipe, the connection pup joint is in a hollow cylinder shape, oneend of the connection pup joint is fixedly provided with an outer sleeve pipe coaxial with the connection pup joint, the inner core pipe is fixedly arranged on the portion, on the inner side of the outer sleeve pipe, of the connection pup joint, the inner core pipe and the outer sleeve pipe are coaxially arranged, an annular connection cavity is formed between the inner core pipe and the outer sleeve pipe, the end of the glass steel pipeline is clamped in the annular connection cavity, and the other end of the connection pup joint is sleeved with a connection sleeve. When the glass steel pipeis repaired, manufacturing of a connector of the pipeline to be repaired, connection between the connector on the pipeline to be repaired and the conversion pup joint and connection between the conversion pup joint and the connection pup joint can be rapidly completed, precious time is saved for on-site urgent repair, and the construction efficiency is improved.