The invention relates to the technical field of natural product deep processing, in particular to a novel extraction method of momordica saponins. The novel extraction method comprises the following steps that 1, dry bitter gourd powder is taken to be dissolved into ethyl alcohol, oscillating and shaking are conducted to enable the mixture to be uniform, the mixture is put into a 60-DEG C water bath kettle to be extracted for 1 hour and then centrifuged to obtain a momordica saponins alcohol extracting solution, the momordica saponins alcohol extracting solution is subjected to vacuum concentration in a rotary evaporator until a concentrated solution is free of alcohol smell, and then a momordica saponins extracting solution A is prepared; 2, extraction is conducted by adopting an isopropyl alcohol-(NH4)2SO4 aqueous two-phase system, after partition equilibrium is achieved, phase separation is conducted, an upper phase is take, and a momordica saponins extracting solution B is prepared; 3, the crude momordica saponins extracting solution B is further purified through AB-8 type macroporous adsorption resin, and a fine momordica saponins extract C is prepared; 4, freeze drying is conducted to prepare the finished momordica saponins. Compared with a traditional method, the momordica saponins prepared through ethanol extraction, aqueous two-phase extraction and macroporous resin separation has the advantages that the yield of the momordica saponins is increased, and the blood glucose reducing activity of the momordica saponins is also obviously improved.