The invention relates to a deodorizing liquid for rabbit culture. The deodorizing liquid is prepared from the following raw materials in parts by weight: 2 to 3 parts of biological zymocyte, 900 to 1100 parts of warm water with a temperature of 50 DEG C, 45 to 55 parts of brown sugar, 90 to 110 parts of corn flour, 45 to 55 parts of cooked soybean meal powder, and 45 to 55 parts of cooked rapeseed meal powder. The manufacturing method comprises steps of material preparation, breeding, mixing, and fermentation. The application method comprises the following steps: (1) weighing a certain amount of prepared deodorizing liquid, diluting the deodorizing liquid with water according to a ratio of deodorizing liquid to water of 1:10, then spraying the diluted deodorizing liquid into rabbit feed, evenly stirring, and feeding the rabbits by the feed; (2) weighing a certain amount of prepared deodorizing liquid, then diluting the deodorizing liquid with water according to a ratio of deodorizing liquid to water of 1:25, and then spraying the diluted deodorizing liquid into rabbit manure which is excreted by the rabbits in the step (1). The invention aims to provide a deodorizing liquid for rabbit culture, a preparation method and application method thereof. The rabbit manure is deodorized through a microbial fermentation method, mosquitoes will not generate around the processed rabbit manure, the rabbit manure can be cleaned for every 3-5 months, the labor resource and material resource are both reduced, and at the same time the manure is effectively used and can be used to produce farm manure.