Provided is a glass location vacuum pad assembly and use method. A glass location vacuum pad is formed by a pad body, an upper vacuum cavity, a lower vacuum cavity, an upper vacuum channel, a lower vacuum channel, an upper connector, a lower connector, a first annular sealing groove, a second annular sealing groove and O-shaped sealing ring pads. The pad body is cast to be of an I-shaped structure, the cylindrical upper vacuum cavity is processed in the middle of the upper portion of the pad body, the cylindrical lower vacuum cavity is processed in the middle of the lower portion, at least two annular sealing grooves are formed in the upper plane and the lower plane of the pad body, and the O-shaped sealing ring pads are installed in the annular sealing grooves correspondingly. The glass location vacuum pad is simple and efficient in structure design, ideal in location and attraction effect, convenient to manufacture, simple in operation, capable of reducing labor intensity of operators, low in manufacture cost, capable of saving rubber raw materials, easy to operate, convenient to maintain, flexible in operation and suitable for popularization and use.