The invention discloses a Helan mountain delicacy hot pot condiment and a preparation method thereof. The hot pot condiment comprises raw materials including tricholoma matsutake enzymolysis juice, boletus concentrate, edible fungus concentrate, rape oil, ginger, white granulated sugar, salt, garlic, gourmet powder and chicken flavor; the zymohydrolysis, abstraction and concentration of edible fungi difficult in digesting in the hot pot condiment are performed, the edible fungi are made into small molecule concentrated nutritious substances which are easy to be absorbed by a human body and have a nourishing effect, and the small molecule concentrated nutritious substances are added to the hot pot condiment. As a result, not only is the taste of the hot pot condiment improved, but also the absorption of the nutritious substances is facilitated, the medicinal and edible effects are remarkable, in addition, burdens of a stomach and intestines can be effectively lowered, appetite is improved, the stomach is protected, particularly, both the raw materials and the preparation method of the hot pot condiment satisfy the process requirements of Muslim foods, Muslim consumers' demand for eating concentrated nutritious hot pot is satisfied, and the industrialization development of a Muslim hot pot condiment is prompted.