The invention discloses tortoise jelly capable of refreshing. The tortoise jelly is prepared from the components of, by weight, 50 to 70 parts of mesona chinensis benth, 40 to 50 parts of a stabilizer, 20 to 40 parts of astragalus membranaceus, 20 to 40 parts of ginseng, 10 to 20 parts of atractylodes macrocephala koidz, 20 to 30 parts of cassia twigs, 20 to 30 parts of liquorice, 10 to 20 parts of rhizoma acori graminei, 10 to 20 parts of herba eupatorii, 20 to 40 parts of radix curcumae, 5 to 10 parts of turtles and 0.1 to 0.5 part sweetening agent. The invention further discloses a preparation method of the refreshing tortoise jelly. The preparation method comprises the steps of killing and cleaning tortoise, adding water, decocting for 3.5 to 5 hours, and filtering to obtain a tortoiseextracting solution; mixing mesona chinensis benth, astragalus membranaceus, ginseng, atractylodes macrocephala koidz, cassia twigs, liquorice, rhizoma acori graminei, herba eupatorii, radix curcumaeand the tortoise extracting solution in proportion, adding water to extract for 2 to 4 times, extracting for 1 to 2 hours each time, and combining the two extracting solutions to obtain a decocted extracting solution; and adding the sweetening agent and the stabilizer into the decocted extracting solution, stirring until the mixture is gelatinized, sub-packaging, and cooking at high temperature to obtain the refreshing tortoise jelly.