The invention belongs to the field of soil improvements, and relates to application of soil conditioner containing chitosan oligosaccharide to acid soil conditioning. The conditioner is prepared from 0.05-100 parts of chitosan oligosaccharide, 0-80 parts of soil improvement components, 0-15 parts of artificially synthesized high-molecular compounds and 0-100 parts of mineral substances. The soil improvement components are one or more of lime, plant ash, dolomite, coal ash, slaked lime, calcium carbonate, powdered rock phosphate, ammonium carbonate, lime nitrogen and the like in alkaline substances. The artificially synthesized high-molecular compounds are obtained by mixing one or more of polyacrylic acid type high-molecular compounds, vinyl acetate maleic acid type high-molecular compounds and polyvinyl alcohol high-molecular compounds. The mineral substances are obtained by mixing one or more of peat, brown coal, decomposed coal, vermiculite, bentonite, zeolite, perlite and sepiolite. When applied, the soil conditioner is uniformly sprayed or atomized on the surface of acid soil, and the acid soil is uniformized through deep tillage arrangement or rotary tillage; or, the soil conditioner is used for topdressing; in the application method, the application amount of the soil conditioner ranges from 50 kg/hm<2> to 1000 kg/hm<2>, the soil conditioner is applied 1-3 times, the pH value of soil can be promoted to rise, the soil acidity can be lowered, or soil can be neutral.