The application discloses an extract of PhIP generation inhibiting
Amomum villosum and a preparation method and application thereof. The preparation method comprises the following steps: (1) crushing dried
Amomum villosum fruits, and mixing the crushed
Amomum villosum fruits with a eutectic
solvent; (2) performing heating and ultrasonic extraction on the mixture, and filtering to obtain a crude extract; (3) purifying the crude extract through a
macroporous resin column, and collecting eluate; and (4) removing
solvent from the eluate, and freeze-
drying to obtain the
Amomum villosum extract. The application solves the problem that there is a lack of a PhIP inhibitor which is efficient, natural, can simultaneously improve
food safety and does not affect the quality of meat products in the prior art. The
Amomum villosum extract prepared by the application can efficiently inhibit PhIP: the
Amomum villosum extract prepared by the application can significantly reduce the content of PhIP in a
roast beef pie and a chemical
model system. In the
roast beef pie, the content of PhIP can be significantly reduced by adding 0.5 mg / g of the Amomum villosum extract, and the Amomum villosum extract has no significant influence on the
roasting loss rate,
hardness, elasticity,
chewiness and cohesiveness of the
roast beef pie.