The invention discloses glue injection equipment. The glue injection equipment comprises an equipment fixed frame, front-and-back adjusting frames, up-and-down adjusting frames, left-and-right adjusting frames, needle cylinder fixing frames and needle cylinders; the up-and-down adjusting frames are installed on the equipment fixed frame, each front-and-back adjusting frame is installed on the corresponding up-and-down adjusting frame, each left-and-right adjusting frame is installed on the front-and-back adjusting frame, each needle cylinder fixing frame is installed on the corresponding left-and-right adjusting frame, each needle cylinder is provided with a needle and installed on the corresponding needle cylinder fixing frame, and each needle cylinder fixing frame is installed on a guide rail on the side portion of the corresponding left-and-right adjusting frame in an up-and-down sliding mode through a sliding block on the side portion. According to the glue injection equipment, the needles can be rapidly aligned to products to be subjected to glue injection, and a lot of time consumed due to a small deviation for adjustment is not needed; the needles can make contact with an upper deviation and keep still in the glue injection process, by means of the sliding blocks and the guide rails, the needle cylinder fixing frames can move upwards along the guide rails automatically, collision deformation or bending of the needles is prevented, and therefore protection on the needles is achieved, and new needles do not need to be replaced frequently.