A multi-channel microfluidic
label-free
pathogen detection
system and method with internal reference correction is disclosed, relating to the field of
pathogen detection. The method includes: acquiring the initial interference spectrum of each functional detection region before the reaction; injecting the sample to be tested and distributing it to multiple parallel microfluidic channels according to a preset flow path; specific binding of the target
pathogen to the
aptamer to form an
aptamer-pathogen complex layer; acquiring the post-reaction interference spectrum of each functional detection region, comparing the post-reaction interference spectrum with the initial interference spectrum before the reaction, and calculating the change in
optical path difference of each functional detection region; calculating the target detection
signal and comparing it with a
standard curve to obtain the concentration of the target pathogen in the sample. This invention achieves continuous, accurate, and repeatable spectral acquisition of multiple functional detection regions, without the need for
nucleic acid amplification or labeling, realizing multi-channel parallel, automated acquisition, internal reference correction, and quantitative analysis of pathogen concentration, thus improving the accuracy and reliability of the detection results.