The invention relates to high-efficiency microbial organic fertilizer prepared from methane slag. The high-efficiency microbial organic fertilizer mainly comprises methane slag, plant growth regulator, microbial functional bacteria, binder and disintegrating agent which are used as active ingredients. The high-efficiency microbial organic fertilizer comprises the following components in percentage by weight: 30.0 to 85.0 percent of methane slag, 0.001 to 3.0 percent of plant growth regulator, 0.1 to 1.0 percent of microbial functional bacteria, 0.5 to 1.0 percent of binder and 3.0 to 10.0 percent of disintegrating agent and the balance of other aids and fillers which are allowable and acceptable in chemical fertilizer and pesticide. The high-efficiency microbial organic fertilizer is abundant in organic matters, amino acid, nitrogen, phosphorus, potassium, trace elements and the proper plant growth regulator, and can obviously improve fertilizer efficiency of the methane slag, reduce the using amount of the methane slag, regulate growth of potatoes, obviously increase the yield of the potatoes and improve the quality of the potatoes. The high-efficiency microbial organic fertilizer is mainly applied to planting of the potatoes, Chinese medicinal materials and vegetables.