An area array detection stimulated radiation depletion imaging system, including an excitation light source for generating excitation light, a depletion light source for generating depletion light, a beam expander system, a vortex phase modulation plate, a dichromatic mirror, and a wave plate , scanning galvanometer, scanning lens, tube lens, objective lens, nano-shift stage, telephoto converging lens and area array detector, the excitation light after beam expansion by the beam expander system and the beam expander system, vortex phase The lost light after beam expansion and phase modulation by the modulation plate enters the dichroic mirror and then enters the wave plate. After scanning the lens, tube lens and objective lens, focus on the inside of the sample at the nano-shift stage, and the optical signal generated by the excitation light and loss light focused on the inside of the sample at the nano-shift stage passes through the detection optical path and the telephoto converging lens Converged on the area array detector for imaging. The application is simple and convenient to operate and has high utilization rate of light energy.