The invention relates to a handheld
fluorescence detector which comprises a clamping groove, a first photoelectric switch, a second photoelectric switch, a
light source, a light
path system, and a
photoelectric sensor, a
signal amplification circuit, a peak holding circuit, a
microprocessor and an analog-to-
digital converter which are connected in order. The first photoelectric switch and the second photoelectric switch are mounted on the clamping groove and sequentially sense the
reagent cards inserted into the clamping groove. The light
path system comprises a first convex lens, a dichroic mirror, a second convex lens, an
optical filter and a third convex lens; and light emitted by the
light source sequentially penetrates through the first convex lens, is reflected to the second convex lens through the dichroic mirror, penetrates through the second convex lens and forms a
light spot on a tested
reagent card, and exciting light sequentially penetrates through the second convex lens, the dichroic mirror, the
optical filter and the third convex lens to reach the
photoelectric sensor. The
detector is provided with a precise optical excitation and collection detection
system, signals of the positions of the
quality control line and the
test line of the
reagent card can be independently collected, the calculated amount is small, the speed is high, and the size of the
detector can be smaller.