The invention relates to the technical field of daily chemical industry, in particular to a mosquito repellent composition, a preparation method and an application thereof. The raw material of the mosquito repellent composition includes the following components in parts by weight: 0.01-1.0 part of Magnolia officinalis extract, 0.01-1.0 part of Aina radix extract, 0.01-2.0 part of Cnidium extract, 0.02-1 part of peppermint extract 2.0 parts, 0.1-2.0 parts of citrus peel extract, 0.5-1.5 parts of Chinese prickly ash extract, and 0.2-1.0 parts of ethanol. The preparation method includes the following steps: respectively adding water to the medicinal materials Magnolia officinalis, Aina incense, mint, citrus peel and Chinese prickly ash, and extracting volatile oil by steam distillation, filtering and concentrating to obtain a concentrated liquid, and mixing the respective volatile oils and the concentrated liquid to obtain the medicinal material extracts Then add 10-25% acetone aqueous solution to Cnidium and extract, filter, and concentrate to obtain Cnidium extract; Finally, each above-mentioned extract and ethanol are mixed uniformly to obtain a composition. The composition not only repels mosquitoes for a long time, but also can relieve itching, reduce inflammation and sterilize.