The invention belongs to the field of control panels of instrument control systems and main control rooms in nuclear power plants, and particularly relates to a physical isolation device and method for a control panel of a nuclear power plant. The device comprises an external wire inlet cable, a wiring terminal set, a closed metal wire groove, a metal hose a, a metal hose b, an isolation keel and panel surface equipment, wherein the external wire inlet cable is connected with the wire inlet end of the wiring terminal set, the wire outlet end of the wiring terminal set is respectively connected with the closed metal wire groove and the metal hose a, the closed metal wire groove is connected with the metal hose b, the metal hose a and the metal hose b are connected with one end of the isolation keel, and the other end of the isolation keel is connected with the panel surface equipment. According to the physical isolation device, by means of the keel and a detachable metal plate, installation space is saved, the problem that a traditional isolation device is difficult in engineering installation is solved, the structural design requirement for a panel body is reduced, the machining and manufacturing period of the panel body can be shortened, and implementation cost is saved.