The invention discloses a lifting mechanism of a frame splitting machine for industrial aquaculture. The lifting mechanism comprises a lifting body, a sliding block body and a lifting screw rod assembly, wherein the lifting body is connected with the sliding block body, the sliding block body is positioned on the lifting screw rod assembly, and the lifting screw rod assembly drives the lifting body to slide up and down through the sliding block body. According to the lifting mechanism, the overall structural design is ingenious and reasonable, a driving motor drives a lifting screw rod to rotate through a gear conversion box, so that the lifting of the sliding block body is achieved to drive the lifting of the lifting body, the step-by-step separation between a lower culture frame and an upper culture frame stack is achieved, the assembly and disassembly of the culture frame stack is more convenient, the assembly line operation of cocoon collection in the culture frame is achieved, thewhole operation is more automated, and the labor intensity of workers is effectively reduced; and the lifting screw type lifting is adopted, the stability in the lifting process of the lifting body can be effectively guaranteed, the lifting process of the lifting body is more smooth and stable, collision caused by swinging of the lifting body in the lifting process is avoided, and the operation safety performance is improved.