The invention relates to a portable tool box for quick clenbuterol, ractopamine and salbutamol detection. The portable tool box for quick clenbuterol, ractopamine and salbutamol detection comprises abox body, a box cover, a water bath, a centrifuge, a colloidal gold reader, and a centrifugal tube box, wherein triple card storage slots are formed in the box body; the box cover is hinged to one endof the box body; and buckles are arranged on the box body far from the end of the box body hinged with the box cover. The portable tool box for quick clenbuterol, ractopamine and salbutamol detectionprovided by the invention has the advantages that the water bath, the centrifuge, the colloidal gold reader, the centrifugal tube box, the triple card storage slots and a power supply device are mounted in the box body of the tool box, so that centrifugal tubes and triple cards, which are required for clenbuterol, ractopamine and salbutamol detection, can be conveniently stored; and as the waterbath, the centrifuge, the colloidal gold reader and other sample treatment and detection devices are mounted, clenbuterol, ractopamine and salbutamol detection can be quickly carried out anywhere as long as the tool box is available.