3results about How to "With self-calibration function" patented technology

A rain gauge with self-calibration function includes a cylindrical body with an open top. A funnel is provided inside the cylinder, dividing the inner cavity of the cylinder into a water collection chamber and a measuring chamber from top to bottom. An ultrasonic probe for detecting water level is installed in the measuring chamber. The cylinder is supported by a mounting bracket, on which a weight sensor for weighing the cylinder is mounted. A calibration pipe and a calibration valve are provided on one side of the measuring chamber, and a drain pipe and a drain valve are provided at the bottom of the measuring chamber. The weight sensor, ultrasonic probe, calibration valve, and drain valve are all electrically connected to a data processing unit. Compared with the prior art, this application has the following beneficial technical effects: it has a self-calibration function, enabling online self-calibration with zero manual intervention and zero external equipment, thus maintaining long-term accuracy in liquid level detection.

The present invention provides an RT-RAA primer probe, a ratio fluorescence chromatography kit and an application thereof for detecting influenza A (H1N1) virus, and belongs to the field of biological detection technology. The RT-RAA primer probe for detecting influenza A (H1N1) virus provided by the present invention can specifically amplify influenza A (H1N1) virus. The present invention combines the isothermal amplification technology mediated by recombinant polymerase with the nucleic acid fluorescence ratio chromatography test strip color development method. The RT-RAA amplification product flows through the test strip, and the product first combines with the red fluorescent microspheres, and then is captured by the T line. The green fluorescent microspheres carried on the T line are gradually covered by the red fluorescent microspheres, showing a characteristic color tone change. The detection personnel can semi-quantitatively determine the concentration of the analyte according to the color tone. It has the advantages of high sensitivity and strong specificity. It can be judged by naked eyes with a small ultraviolet flashlight. It does not require complex instruments and equipment and is suitable for rapid on-site detection.

This invention discloses a method for preparing a plasmonic-enhanced fluorescence-coupled aptamer ratio sensor based on gold nanoparticles and its application in the visual detection of amyloid protein. The invention involves preparing gold nanoparticles and immobilizing them on an amino-modified ELISA plate by adding EDC / NHS. β-amyloid aptamers, with amino and thiol groups modified at both ends respectively, are grafted onto the gold nanoparticle-modified ELISA plate via Au-S bonds. EDC / NHS is then added to graft Rosa Bengal onto the aptamers on the gold nanoparticles via amide bonds, resulting in a plasmonic-enhanced fluorescence-coupled aptamer as the responsive fluorescent probe and 9-anthracarboxylic acid as the reference fluorescence, thus creating a plasmonic-enhanced / ratio-fluorescent probe sensor. The plasmonic-enhanced / ratio-fluorescent probe sensor prepared by this invention exhibits good stability, enabling portable, ultrasensitive, and specific visual quantitative detection of β-amyloid protein.