The invention discloses a tinospora sinensis pain-relieving tincture which is prepared from the following 29 pure Chinese herbal medicines: gastrodia elata, notopterygium root, radix angelicae pubescentis, ovate leaf holly bark, radix stephaniae tetrandrae, semen coicis, ligusticum wallichii, resurrection lily rhizome, ephedra, beautiful sweetgum fruit, caulis spatholobi, tinospora sinensis, red flower, peach kernel, cotex dictamni, curcuma zedoary, rhizoma sparganii, achyranthes root, the root of kudzu vine, teasel root, prepared myrrh, prepared frankincense, radix notoginseng, akebiaquinata, white paeony root, corydalis tuber, motherwort, parasitic loranthus and rhizoma nardostachyos. The preparation method comprises the following steps: sealing and soaking the pure Chinese herbal medicines in 75% alcohol for 30-35 days, filtering and filling so as to obtain the tincture, so that the drug effect is fully achieved, and the tincture is scientific and reasonable in formula and easy to prepare. The inventor works on the traditional Chinese medicine for thirty years, and according to clinical verifications of over ten thousand of patients, the tinospora sinensis pain-relieving tincture is a pain-relieving tincture with the advantages of fast effectiveness, fast pain-relieving effect, diversified functions and wide adaptability and without any additive, preservative, Western medicine analgetic or toxic or side effect.