The invention provides an isolation window for preventing and controlling cross infection of pharynx/nose test sample. The isolation window comprises a disinfection cavity, an induction window, an annular belt-shaped filter membrane, isolation cloth and an air suction pipe with an inlet covered by the filter membrane, the filter membrane longitudinally surrounds the air suction pipe and is supported and driven by a roller to circularly rotate and disinfect in the disinfection cavity, a detection channel is arranged under an inlet of the air suction pipe, the induction window is arranged above the front end of the detection channel, the induction window is located right in front of the inlet section of the air suction pipe, the isolation cloth covers the induction window, extends into the disinfection cavity, circularly rotates in a disinfection cabinet under supporting and driving of the roller and performs disinfection, and surrounds the inlet section of the air suction pipe in the horizontal direction, a disinfection cavity exhaust fan is arranged at the upper part of the disinfection cavity, and suction is used through a fan to keep the micro-negative pressure at a gap where the filter membrane and the isolation cloth enter the disinfection cavity. According to the isolation window, a head of an examinee can be accurately positioned, the sampling efficiency is high, gas exhaled by the examinee can be sucked out, filtered and disinfected in a short distance, disinfection is thorough, safety and reliability are achieved, and the isolation window is compact in structure.