The invention discloses a simulation experiment device for carbon isotope analysis in a shale gas release process. The device comprises a reaction chamber, a temperature maintaining part, a vacuumizing part, a monitoring part, a filling part, a water injection part, a gas collecting part, a pressure balancing part and an adjusting component, the temperature maintaining component wraps the reactionchamber, the vacuumizing component, the monitoring component, the filling component, the water injection component, the gas collecting component and the pressure balancing component are connected with the reaction chamber, and the adjusting component fixes the reaction chamber and the pressure balancing component. With the adoption of the manner, an experiment can be carried out under a closed condition, the temperature required by each stage is maintained, and the pressure change is monitored; the fluid environment in the shale gas release process is simulated; the released shale gas can bestored in a sealed manner, and the released shale gas sample can be quantitatively extracted at any time; and the device is suitable for experiments of shale samples with small granularity, and can beused for experiments of various shale samples with various granularities.