The invention discloses a die-casting mechanism of a horizontal freezing chamber vacuum die-casting machine. The die-casting mechanism comprises a pressure chamber, a die-casting die, a vacuum valve, a punch, an injection rod, a first stop valve, a first vacuum system, a second stop valve, a second vacuum system, a control device and a displacement sensor. The die-casting die comprises a fixed die body and a movable die body, the movable die body and the fixed die body are matched, and therefore a cavity, an inner gate and an exhausting way are formed. The vacuum valve is arranged in a valve cavity in the fixed die body. The punch is fixedly connected with the injection rod. The pressure chamber is provided with a casting gate and a pipe connecting joint, the pipe connecting joint is connected with a second vacuum pipe, the other end of the second vacuum pipe is connected with the second stop valve, and the second stop valve is connected with the second vacuum system. The control device is used for controlling operation of the first vacuum system and the second vacuum system and controlling opening and closing of the first stop valve and the second stop valve. The die-casting mechanism has the beneficial effects of being good in vacuumizing effect, easy and convenient to arrange and maintain, low in fault rate and long in service life.